| Rank | Surname | Given | Residence | Enlistment | Muster | Remarks |
| Sergeant Major | Horne | Daniel H. | Topeka | Aug 20, '62 | Sept. 13, '62 | Promoted Captain 4th Indian regt. June ---, '63. |
| Sergeant Major | Greer | James E. | Topeka | Aug. 19, '62 | Sept. 15, '62 | Promoted Adjutant November 2, 1863. |
| Sergeant Major | Isbell | Isaac H. | Wabaunsee | Aug. 21, '62 | Sept. 13, '62 | Mustered out Aug. 19, 1865; commissioned 1st Lieutenant and Adjutant, vice Taber mustered out, but never mustered. |
| Quartermaster Sergeant | Harper | Robert J. | Manhattan | Aug 25, '62 | Sept. 13, '62 | Promoted Commissary October 8, 1863. |
| Quartermaster Sergeant | Marple | Silas H. | Burlingame | Aug 22, '62 | Sept. 15, '62 | Promoted 1st Lieut. 65th U.S. Colored Troops, January 18, 1864. |
| Quartermaster Sergeant | Cowan | William H. | Topeka | Sept. 22, '62 | Sept. 22, '62 | Mustered out August 19, 1865. |
| Commissary Sergeant | Waterhouse | Robert J. | Jackson Co. | Aug. 16, '62 | Aug. 30, '62 | Reduced to ranks March 9, 1865. |
| Commissary Sergeant | Paine | Charles E. | Emporia | Aug. 23, '62 | Sept. 13, '62 | Mustered out August 19, 1865. |
| Saddler Sergeant | Barnes | James H. | Manhattan | Aug. 28, '62 | Sept. 15, '62 | Mustered out August 19, 1865. |
| Hospital Steward | Ashbaugh | Armenius | Topeka | Aug. 19, '62 | Sept. 13, '62 | Reduced and assigned to Co. E, Nov. 18, 1862. |
| Hospital Steward | Johnson | David H. | Garnett | Aug. 23, '62 | Sept. 11, '62 | Mustered out August 19, 1865. |
| Hospital Steward | Koentz | John P. | Vienna | Sept. 8, '62 | Sept. 15, '62 | Mustered out August 19, 1865. |
| Chief Bugler | Horton | Nathaniel D. | Manhattan | Aug. 25, '62 | Sept. 13, '62 | Mustered out August 19, 1865. |
| Veterinary Surgeon | Barbar | Thomas | Emporia | Aug. 18, '62 | Sept. 10, '62 | Mustered out August 19, 1865. |